flowDensity.Rmd
flowDensity
gates can be converted to CellEngine gates. The class of a flowDensity
gate is "CellPopulation"
. The
flowDensity@filter
is always a polygon, so
cellengine::createPolygonGate
can be used for this
purpose:
file = "sample1.fcs"
flowDensityGate <- flowDensity::flowDensity(file, params)@filter
experimentId <- "5d2f8b4b21fd0676fb3a6a8c"
polyGate <- createPolygonGate(
experimentId,
colnames(flowDensityGate)[1],
colnames(flowDensityGate)[2],
name,
vertices = list(flowDensityGate),
)
As a function:
convertFlowDensity <- function(flowDensityOutput, experimentId, name = NULL, ...) {
flowDensityGate <- flowDensityOutput@filter
createPolygonGate(
experimentId,
colnames(flowDensityGate)[1],
colnames(flowDensityGate)[2],
name,
vertices = list(flowDensityGate),
...
)
}